School of Pharmaceutical
Sciences of Ribeirão Preto

University of São Paulo

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Flow Cytometry Center


The Faculty of Pharmaceutical Sciences of Ribeirão Preto - USP has an Analytical Center for phenotyping of cells from human, animals or microorganisms. It is named Flow Cytometry Center. The equipment is a Flow Cytometer BD FACSCanto I that was donated by FAPESP through a multi-user project (EMU-04 / 09448-5), coordinated by Dr Lúcia Helena Faccioli, and with participation of other professor  from FCFRP-USP. The technical responsible of Flow Cytometry Center is Mrs Fabiana de Morais Rossetto.


Equipment: Flow cytometer BD FACSCanto I


This machine has two lasers, a Blue and another Red, and 6 fluorescence detectors. Below are shown some of the possible fluorochromes and probes can be used.


Flow cytometer BD FACSCanto I - FCFRP-USP

EXCITING

DETECTORS

FLUOROCHROMES and PROBES

BLUE LASER 488 nm

530/30

FITC, ALEXA FLUOR 488, GFP, DAF, CFSE, DCFHDA

BLUE LASER 488 nm

585/42

PE, PI, DHE, TMRE

BLUE LASER 488 nm

670

PERCP, PERCPCY5.5, PECY5, PI, HFS

BLUE LASER 488 nm

780/60

PECY7

 

 

 

RED LASER 633 nm

660/20

APC, ALEXA FLUOR 647

RED LASER 633 nm

780/60

APCCY7



USERS


The Analytical Center serves preferably teachers and students from FCFRP-USP, but when there is availability in the times, its use is open to students and lecturers from other faculties.


PAYMENT


All users pay for the analysis. The money is used for preventive and corrective maintenance of the equipment. The payment is annual and the value depends on the number of users and samples analysed in each period. Payment is made at the Treasury of FCFRP-USP that provided a receipt. 

In addition to the payment for the analyses, the user must bring a gallon of 20 litres of Isogen every 400 tubes analysed, A4 paper for printing the results, and a CD to record the data. It is not allowed to use "pen drive".


BUSINESS HOURS


Monday through Friday: 
8:00 to 12:00 am  - 13:00 to 17: 00 pm


SCHEDULE AND INFORMATION


The users should be scheduled by phone +551633154276 in business hours, with Mrs Fabiana. The cancelled must be notified 24 hours in advance. More information can be requested on the phone up.


ADDRESS


The Flow Cytometry Center is located on the 3rd floor, room number 093-A, Block S, at Street Ruy Barbosa Roselino, School of Pharmaceutical Sciences, Campus of Ribeirão Preto, São Paulo University.


COMPOSITION OF THE USERS COMMITTEE


The Flow Cytometry Center has a commission to regularly evaluate the quality of services provided. Members of the committee are all researchers and users of FCFRP-USP:

Prof. Dr. Fabiola Attié de Castro - DACTB Lecturer
Prof. Dr. Clovis José Prado - DACTB Lecturer
Prof.. . Dr. Maria Lusiane Bendhack - DFQ Lecturer
Prof.. . Dr. Cristina Ribeiro de Barros Cardoso - DACTB Lecturer
Fabiana Albani Zambuzi - PhD student of Bioscience Applied to Pharmacy