School of Pharmaceutical
Sciences of Ribeirão Preto

University of São Paulo

LANGUAGES: | INTRANET

Instrumental Pharmaceutics Lab.

The Instrumental Pharmaceutics Laboratory, LAFAI, was designed and founded in FCFRP / USP in order to provide fundamental physical and chemical analysis services for pre-formulation studies and the characterization of pharmaceutical forms as well as food, polymers and chemicals in general. The services offered to internal and external researchers to FCFRP / USP include thermal analysis by DSC, TGA, DTA, DTG and DTMA, rheological characterization by controlled shear and oscillatory tests, determination of texture by texturometer, obtaining water activity and isotherms DDI water adsorption. 

The LAFAI is coordinated by Drs. Luis A. P. Freitas, Osvaldo de Freitas and Juliana M. Marchetti.

 

THERMAL ANALYSIS


LAFAI is equipped with thermal analysis equipment from Shimadzu (Shimadzu Inc, Tokyo, Japan), models DSC50, TGA50, DTA50, DTG50 and DTMA50. The set comprises data acquisition software and processing TA-50WSI data for analysis in "real-time" graphical presentation of data defining the parameters of analysis and manipulation of data by such techniques as "smoothing", baseline correction, calibration temperature and heat, zero adjustment, calibration expansion and integration derived 1st and 2nd, allowing wide flexibility in analyzing the results. The system also offers various accessories for wider range of applications, such as gas flow control (nitrogen, helium or oxygen), cooling / heating controlled in temperature range from -150 ° to 400 ° C.

APPLICATIONS:

DSC and DTA measures physical changes such as melting or glass transition, chemical reactions, formulations interactions in various materials, superconductors and for the synthesis of compounds at high temperatures.

TGA and DTG – measures the thermally induced weight loss of foods, pharmaceuticals and chemical products, vegetable oils, mixtures of analyzes, oxidation reactions studies and reduction, evaluation of polymer blends, fuel additives, drug stability and antioxidant activity.

DTMA - Determination of glass transition temperatures and secondary transitions to polymers relaxation kinetics, polymer miscibility and gelation and crosslinking temperatures.


RHEOLOGY AND TEXTURE

The LAFAI is prepared for a complete characterization of viscosity and rheological behavior of raw materials and formulated products in the fields of medicines for humans and animals, food, pesticides and many other chemical products. Among the analysis that can be performed are:

1) Viscosity with Ostwald and Hoppler viscometers;

2) the rheological behavior by shear rheometer Brooksfield R/S Plus with Rheocalc software, determining all test parameters and measurement values; yield stress, viscosity (cP P mPa • s, Pa • s, cSt mm² / s) shear stress (dynes / cm² N / m², Pa), shear rate (s-1), torque (%) Speed ​​(rpm) in continuous rotational assays using plate and cone device, precise control of temperature (°C/°F) by Peltier system, graphical plot of the measurements in real time. Calculations on mathematical models, Casson, Bingham, Power Law and flow rate. Calculation of Thixotropy index.

3) Analysis of all rheology oscillatory flow and pharmaceuticals, cosmetics and food by an hybrid Discovery 2 Rheometer HR-TA Instruments.

 

4) Analysis  of texture by a TA.XT Plus Texture Analyzer (Stable Micro Systems) capable of evaluating physical properties such as hardness, adhesion, extensibility, among other, pharmaceuticals, cosmetics and foods. The equipment has different probes for compression tests and tension and has load cells 5kgf and 50 kgf.


 

WATER ACTIVITY AND ADSORPTION/DESORPTION ISOTHERMS

The LAFAI is equipped with equipment to generate isotherms of adsorption/desorption of water by DDI technique (dynamic dewpoint isotherm). This Decagon instrument generates isotherms for water activity measurements and gravimetry in a continuous process of increase/decrease of the sample moisture. The isotherms have wide application in the fields of medicine, food and natural products and are essential in the development of formulations, estimated shelf life, and quality control. The main applications are the determination of: 1) moisture in balance with the environment; 2) critical water activity for; 3) drying tuning parameters; 4) shelf life and stability of the products; 5) balance of moisture to mixtures of components; 6) the degree of crystallinity or amorphous content; 7) the relationship between water activity and glass transitions; 8) the relationship between water activity and temperature of crystallization and 9) hysteresis adsorption / desorption.

 

To book analysis and cost information, contact Aurea D. Lanchote by calling (16) 3602-4430 or email aurea@fcfrp.usp.br.