DNA Sequencing Facility
The DNA sequencing facility of the School of Pharmaceutical Sciences of Ribeirão Preto was started in 2012 unde the direction of Professor Sergio Akira Uyemura. The equipment was donated by FAPESP through multiuser project (EMU 2009/08470-0) and by Research Center for Natural and Synthetic Products. Since this date, the facility has developed the ability to sequence genes, which become a pervasive and essential tool for research in several areas.
Sanger DNA sequencing is carried out on an Applied Biosystems 3500 XL DNA Analyzer and the Next-Generation Sequencing service has a GS Junior/454 (Roche) and an Ion Torrent PGM (Applied Biosystems) and soon an Illumina MiSeq (December/2015). The GS Junior and Ion Torrent sequencers are suitable for smaller projects and faster turnaround times. The MiSeq sequencer will be purchased to offer Illumina's high throughput, short read technology to our researchers.
Facility staff have expertise in most aspects of DNA sequence analysis and can offer help and advice to both experienced researchers and to beginners. Our Facility offers sequencing services to anyone, we are not limited to only Departmental or University users.
Samples must be purified before being sent to the sequencing and must be diluted with ultra pure water or 10 mM Tris- HCl. The presence of EDTA, primers and nucleotides debris can impact the quality of the sequencing .
Schedule and information
The users should be scheduled by phone +55(16) 3315-4199 in business hours, with Mr. Emerson Santos. We recommend that you contact us prior to submitting your samples so that we may best address your specific scientific needs.
The DNA sequence facility is located on the 1st floor, room number 100-A, Block A, School of Pharmaceutical Sciences, Campus of Ribeirão Preto, São Paulo University.
Akira Uyemura, PhSc
Ana Lucia da Costa Darini, PhSc
Monica Tallarico Pupo, PhSc